Excision Repair Characteristics of recB − res − and uvrC − Strains of Escherichia coli
نویسندگان
چکیده
منابع مشابه
uvrC gene function in excision repair in toluene-treated Escherichia coli.
We have examined the role of the uvrC gene in UV excision repair by studying incision, excision, repair synthesis, and DNA strand reformation in Escherichia coli mutants made permeable to nucleoside triphosphates by toluene treatment. After irradiation, incisions occur normally in uvrC cells in the presence of nicotinamide mononucleotide (NMN), a ligase-blocking agent, but cannot be detected ot...
متن کاملCloning of the uvrC gene of Escherichia coli: expression of a DNA repair gene.
We have cloned the uvrC gene of Escherichia coli, using an F' plasmid carrying the uvrC region as a source of DNA. Two plasmids, pSC101 and pBR322, were used as cloning vectors. The recombinant plasmids were selected for their ability to complement the uvrC defect of E. coli strains AB1884 and N177. We conclude that the uvrC structural gene is contained in a 1.9-kilobase DNA fragment. The prote...
متن کاملRole of DNA polymerases in excision repair in Escherichia coli.
The excision repair process following ultraviolet irradiation has been fractionated into its individual steps in toluene-treated cells. Incision can be examined in vitro independently of other reactions by omission of the deoxyribonucleoside triphosphates which prohibits repair synthesis and causes incisions to accumulate. Incision requires ATP, continues from 10 to 15 min at 37”, and is specif...
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Eight virulence factors associated with uropathogenic Escherichia coli (UPEC) were investigated in 204 clinical isolates of E. coli recovered from urine cultures at counts ≥10(5). The bacteria were classified into two groups according to the number of leukocytes in urine samples from which they were isolated: group I ≤8 leukocytes/hpf, 104 strains; group II >8 leukocytes/hpf, 100 strains....
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The aim of the present study was to determine biological characteristics such as expression of fimbriae, Congo red binding, production of hemolysin and aerobactin, adhesion to HeLa and uroepithelial cells and invasion of HeLa cells by Escherichia coli isolates obtained from patients showing clinical signs of urinary tract infection (UTI). Also, the presence of genes (apa, afa, spa) for fimbria ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.112.3.1237-1246.1972